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cd25 cell surface markrs  (Bio-Rad)


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    Structured Review

    Bio-Rad cd25 cell surface markrs
    Figure 2. Flow cytometric selection of CLIP antigen-specific T-cell subsets. Splenic T lymphocytes harvested on day 14 after cessation of cyclosporine treatment were subjected to 3-color immunofluorescence. The cells were stained with the biotinylated soluble MHC class II immunoglobulin construct loaded with the N- or C-terminal CLIP variants (counterstained with CyChrome/streptavidin) and with fluorescein isothiocyanate (FITC)–and phycoerythrin (PE)–conjugated antibodies to <t>CD8</t> and <t>CD25,</t> respectively. The percentage of cells staining with the construct is shown in the histogram; the dot plot displays CD8 and <t>CD25</t> <t>expression</t> of the cells reacting with the peptide-loaded construct.
    Cd25 Cell Surface Markrs, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd25 cell surface markrs/product/Bio-Rad
    Average 93 stars, based on 88 article reviews
    cd25 cell surface markrs - by Bioz Stars, 2026-05
    93/100 stars

    Images

    1) Product Images from "Functional divergence of antigen-specific T-lymphocyte responses in syngeneic graft-versus-host disease."

    Article Title: Functional divergence of antigen-specific T-lymphocyte responses in syngeneic graft-versus-host disease.

    Journal: Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

    doi: 10.1016/j.bbmt.2004.05.003

    Figure 2. Flow cytometric selection of CLIP antigen-specific T-cell subsets. Splenic T lymphocytes harvested on day 14 after cessation of cyclosporine treatment were subjected to 3-color immunofluorescence. The cells were stained with the biotinylated soluble MHC class II immunoglobulin construct loaded with the N- or C-terminal CLIP variants (counterstained with CyChrome/streptavidin) and with fluorescein isothiocyanate (FITC)–and phycoerythrin (PE)–conjugated antibodies to CD8 and CD25, respectively. The percentage of cells staining with the construct is shown in the histogram; the dot plot displays CD8 and CD25 expression of the cells reacting with the peptide-loaded construct.
    Figure Legend Snippet: Figure 2. Flow cytometric selection of CLIP antigen-specific T-cell subsets. Splenic T lymphocytes harvested on day 14 after cessation of cyclosporine treatment were subjected to 3-color immunofluorescence. The cells were stained with the biotinylated soluble MHC class II immunoglobulin construct loaded with the N- or C-terminal CLIP variants (counterstained with CyChrome/streptavidin) and with fluorescein isothiocyanate (FITC)–and phycoerythrin (PE)–conjugated antibodies to CD8 and CD25, respectively. The percentage of cells staining with the construct is shown in the histogram; the dot plot displays CD8 and CD25 expression of the cells reacting with the peptide-loaded construct.

    Techniques Used: Selection, Staining, Construct, Expressing



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    Bio-Rad cd25 cell surface markrs
    Figure 2. Flow cytometric selection of CLIP antigen-specific T-cell subsets. Splenic T lymphocytes harvested on day 14 after cessation of cyclosporine treatment were subjected to 3-color immunofluorescence. The cells were stained with the biotinylated soluble MHC class II immunoglobulin construct loaded with the N- or C-terminal CLIP variants (counterstained with CyChrome/streptavidin) and with fluorescein isothiocyanate (FITC)–and phycoerythrin (PE)–conjugated antibodies to <t>CD8</t> and <t>CD25,</t> respectively. The percentage of cells staining with the construct is shown in the histogram; the dot plot displays CD8 and <t>CD25</t> <t>expression</t> of the cells reacting with the peptide-loaded construct.
    Cd25 Cell Surface Markrs, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd25 cell surface markrs/product/Bio-Rad
    Average 93 stars, based on 1 article reviews
    cd25 cell surface markrs - by Bioz Stars, 2026-05
    93/100 stars
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    Figure 2. Flow cytometric selection of CLIP antigen-specific T-cell subsets. Splenic T lymphocytes harvested on day 14 after cessation of cyclosporine treatment were subjected to 3-color immunofluorescence. The cells were stained with the biotinylated soluble MHC class II immunoglobulin construct loaded with the N- or C-terminal CLIP variants (counterstained with CyChrome/streptavidin) and with fluorescein isothiocyanate (FITC)–and phycoerythrin (PE)–conjugated antibodies to CD8 and CD25, respectively. The percentage of cells staining with the construct is shown in the histogram; the dot plot displays CD8 and CD25 expression of the cells reacting with the peptide-loaded construct.

    Journal: Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

    Article Title: Functional divergence of antigen-specific T-lymphocyte responses in syngeneic graft-versus-host disease.

    doi: 10.1016/j.bbmt.2004.05.003

    Figure Lengend Snippet: Figure 2. Flow cytometric selection of CLIP antigen-specific T-cell subsets. Splenic T lymphocytes harvested on day 14 after cessation of cyclosporine treatment were subjected to 3-color immunofluorescence. The cells were stained with the biotinylated soluble MHC class II immunoglobulin construct loaded with the N- or C-terminal CLIP variants (counterstained with CyChrome/streptavidin) and with fluorescein isothiocyanate (FITC)–and phycoerythrin (PE)–conjugated antibodies to CD8 and CD25, respectively. The percentage of cells staining with the construct is shown in the histogram; the dot plot displays CD8 and CD25 expression of the cells reacting with the peptide-loaded construct.

    Article Snippet: The shaded area, however, repr etween N- and C-terminal flanking domain dependence. eric molecule loaded with unrelated peptides was [ B & M T neffective in isolating CLIP-specific T cells [23]. low cytometric multicolor analysis was used to isoate the antigen-specific T cells and to confirm expresion of the CD4, CD8, and CD25 cell-surface markrs (phycoerythrin- or fluorescein isothiocyanate– onjugated mouse anti-rat CD8, anti-CD4, and CD25 onoclonal antibodies; Serotec, Harlan Bioproducts or Science, Indianapolis, IN).

    Techniques: Selection, Staining, Construct, Expressing